Fabio Romerio of the University of Maryland, Baltimore in the U.S. seeks to identify surface biomarkers of HIV latently infected cells by comparing membrane proteomes of latently infected and uninfected cells. Unique biomarkers allow selective killing of latently infected cells with specific ligands, or targeted delivery of chemotherapeutic agents.

Joao Goncalves of ADEIM - Faculdade de Farmacia Lisboa in Portugal proposes to develop molecular sensor strategies using nanoparticles to target memory T cells to deliver a toxin that will be expressed when zinc-finger proteins detect HIV-1 sequences.

Jerome Zack of the University of California, Los Angeles in the U.S. will test nanoparticles for their ability to specifically activate HIV from latently infected cells. Once the latent cells express viral proteins, host immune responses and targeted therapies can be used to kill the cell. If successful, this project could provide an effective approach to eliminate an important viral reservoir from HIV infected individuals.

Marcos Fioravanti and Chris Canaday of Fundación In Terris in Ecuador will develop an easy-to-use urine-diverting dry toilet that uses a pedal to "flush" feces into a pipe and mix the waste with dry material for faster composting. Following toilet use, pushing the pedal turns an auger in the pipe, withdrawing the waste to eliminate odors and to enable safe sanitation, after which it can be used as fertilizer. In Phase I, they designed, built and laboratory-tested three prototypes with varying designs. The two best designs, one for home use and one for public use, were field tested in Ecuador to evaluate performance and usability. In Phase II, Fioravanti, now working with co-investigator Chuck Henry, will further refine the design through feedback from the field, and test modified prototypes with a view to conduct large-scale local demonstrations across different continents to promote user acceptance. They will also look to scale-up manufacturing to low-cost mass production.

Virginia Gardiner of Loowatt Ltd. in the United Kingdom will develop a waterless toilet that uses biodegradable film to safely separate and seal waste into a portable cartridge for local anaerobic digestion. The digester will produce fuel (biogas) and fertilizer, creating local waste treatment economies. In Phase I Gardiner performed market research in Madagascar to adapt the basic toilet system to local contexts and to develop a business model. She further developed the toilet, digester, and post-treatment system, and built a pilot system that was tested using human waste in the laboratory. In Phase II, Loowatt looks to scale-up their technology and tools for the global market by further developing the anaerobic digester, readying toilet components for mass production and local manufacture and supply, and exploring local manufacturing possibilities for the biodegradable film.

Steve Lindsay of the London School of Hygiene and Tropical Medicine in the United Kingdom seeks to demonstrate that flies are transmitters of diarrheal diseases due to their attraction to fecal matter and to food sources, and proposes to design traps that attract, capture and kill flies in latrines. If successful, the reductions in flies may reduce diarrheal diseases in local communities.

Bin Fan of the Research Center for Eco-environmental Sciences in China seeks to develop a decentralized sanitation system which uses a low-cost waterless, vacuum system to collect excrement and kitchen waste. The combined waste can then be processed into organic fertilizer.

Joe Schneider of LAAMScience in the U.S. will develop a reusable and self-decontaminating menstrual napkin that uses photodynamic dyes to inactivate microbial growth on the fabric. The napkin would prevent the need for expensive disposable pads and tampons while offering a hygienic alternative that self-disinfects in the absence of soap or clean water.

Mumtaz Arthur and colleagues of Biofilcom Ltd. in Ghana will develop and field test a prototype toilet facility that incorporates an aerobic digester to decompose waste along with a low-cost microflush valve that uses minimal amounts of wastewater from the washbasins to improve sanitation and user experience. The field tests will help assess and refine cultural, sanitation, and financial aspects of these community facilities. In Phase I, Stephen Mecca of Ghana Sustainable Aid Project in the U.S., along with partners in Ghana, installed and evaluated the performance of three prototypes in Ghana: a 9-stall public toilet, a 3- to 5-stall school toilet, and a 1- to 2-stall family toilet. They demonstrated that the toilet system was effective at removing odors, and isolating and digesting waste, with low associated costs. They also conducted a field survey to evaluate sanitation habits, health and impacts on potential users, and researched ways to improve some of the associated subsystems such as harvesting rainwater for use in the washbasins. In Phase II, Mumtaz Arthur of Biofilcom Ltd. in Ghana will scale up field-testing to an entire village by installing 400 stalls, further research the digestion process, and work to produce a next generation low-cost prototype for local manufacturing, supply, and easy assembly.

Gary Foutch and AJ Johannes of Oklahoma State University in the U.S. propose to develop a small-scale device in which an auger forces feces and other solid wastes device through a die that results in high temperatures and pressure that dewaters the waste and destroys microorganisms. The device could reduce odor, insects, surface and ground water contamination, and the associated spread of diseases. In Phase I, they designed a laboratory-scale extruder, which is a fixed shell containing a rotating inner core driven by a motor, and an air-driven plunger to push in the material and generate pressure. They tested its performance using different operating conditions. Preliminary results revealed that their method could destroy 99 percent of parasitic worm eggs in baboon feces. In Phase II, Foutch, AJ Johannes and colleagues will develop and field test a next-generation stand-alone extruder that can effectively sanitize different types of solid sludge in the field, and is also capable of water recovery via evaporation. They will also design a sanitation module from their extruder that can be incorporated into the Omni-Ingestor technology, which is a modular system that combines sanitation with waste removal and transportation.

Haim Breitbart of Bar-Ilan University in Israel will design and test antisense molecules that will inactivate specific genes within sperm that are essential to the fertilization process. If successful, these antisense molecules can be used to develop a reversible oral male contraceptive.