Haiqing Sheng and collaborators Carolyn Bohach and Scott Minnich from the University of Idaho in the U.S. will exploit the CRISPR-Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR-associated protein 9) system in a dual approach to combat enteropathogenic Escherichia coli (EPEC) infections. EPEC cause diarrhea and result in several hundred thousand infant deaths annually. They will engineer a CRISPR-Cas9 cassette to recognize and cleave a DNA sequence found specifically in EPEC, which will lead to selective EPEC cell death in the intestinal tract without affecting other beneficial bacteria. The engineered CRISPR-Cas9 will be administered using both safe EPEC-targeting viruses (bacteriophage) to treat primary infections as well as probiotic E.coli to stably establish the EPEC-targeting plasmid in the gut and block re-infection. They will test it using an established rabbit model of EPEC infection, which will pave the way for future clinical testing.

Henrique Silveira of the Instituto de Higiene e Medicina Tropical (IHMT) in Portugal and colleagues have identified a peptide in human blood that promotes female mosquito reproduction. They will test whether it can be added to artificial diets to improve mosquito breeding in the laboratory for studying vector-borne diseases like malaria. The human peptide activates a so-called G protein-coupled receptor in the mosquito, which somehow triggers reproduction. They will study the mechanism further, and use the most active form of the peptide to formulate an artificial meal and test its effect on different aspects of mosquito reproduction compared to a normal human blood meal.

Stan Stalnaker of Hub Culture Services Ltd. in the United Kingdom will provide the global poor, merchants and partner NGOs with easy access to digital currency for making and receiving payments or distributing aid, with no transaction costs using mobile phones. The digital currency can be exchanged for a local currency for a fee using a Gateway Broker, who can be any merchant, NGO or bank. They will develop a new open-source set of APIs (application programming interface), and simple consumer and merchant interfaces using mostly icons so that people can easily register and transfer payments.

Daniel Swale of Louisiana State University Agricultural Center in the U.S. will develop a trap to attract and kill pregnant Anopheles mosquitoes and their larvae, which transmit malaria. They will identify the best compound for attracting pregnant females based on either the known attractant Cedrol, a sugar-based attractant, or CO2. They will also test whether the larval development inhibitor triflumuron, alone or in combination with a potassium ion channel inhibitor, can be effectively transferred to the traps by mosquitoes from resting chambers, and destroy the residing larvae. The most promising combinations will be evaluated in a semi-field environment with the common malaria mosquito in southeast United States.