Low-Cost All-Membrane Process to Purify MAM01 Antibodies from C1 Cell Lines

Cristiana Boi with Ruben Carbonell of North Carolina State University in the U.S. will develop a purification system for the antimalarial monoclonal antibody MAM01 that uses an all-membrane chromatography process with single-use membranes made from low-cost nonwoven materials. This system will be combined with a protein expression system using the thermophilic filamentous fungus Thermothelomyces heterothallica C1 to create a low-cost biomanufacturing platform. Collaborating with Dyadic International, they will obtain MAM01-containing supernatants from the expression system and analyze its components to guide development of the purification system. Based on these results, small-scale purification experiments will be performed, testing suitable membrane-coupled ligands, membrane configurations, purification conditions and steps, and integration of the system into single-use cassettes. They will also determine the process requirements to scale up the platform.