Identification and Characterization of Vector-Borne Pathogens and Vector Exposures to Define Regional Biomonitoring Strategies and Vector Control Efforts in Cambodia
Jessica Manning and Fabiano Oliveira of the National Institute of Allergy and Infectious Diseases along with Daniel Parker of the University of California Irvine, all in the U.S., will develop a combinatorial approach to better define the incidence of vector-borne diseases in Cambodia in order to limit their transmission. The global incidence of vector-borne diseases such as malaria and dengue are increasing globally. However, accurately defining the disease-burden is challenging, particularly in resource-poor settings where diagnostics and expertise are limited. They will perform an exploratory clinical study in a peri-urban referral hospital with a catchment area of over 60,000 people in Kampong Speu Province, Cambodia, using a combination of approaches to define the high-resolution landscape of vector-borne diseases in the heart of the Greater Mekong Subregion. The study will involve next generation sequencing to identify pathogens in prospectively collected samples from infants with fever in their existing pediatric cohort as well as in new samples to be collected from febrile individuals aged between 0 and 45 arriving at the hospital; ELISA to generate antibody reactivity profiles of vector saliva, which plays a critical but often overlooked role in disease transmission; and geolocating cases using open-source (Quantum GIS) software. These data will be used to model transmission risk and identify target areas for interventions.